KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation

is feasible, with CDK12 absence possibly signifying defective DDR function. reduced expression of a number of DDR proteins including ATR, Ku70/Ku80,

KU70 (-/-) and DNA-PKcs (-/-/-)chicken DT40 cells are reportedly highly sensitive to the DNA topoisomerase II inhibitor etoposide. Here we report that KU70 and DNA-PKcs unexpectedly function together during the induction of apoptosis after exposure to high levels of etoposide. In the presence of 100 microM etoposide, apoptosis was induced within 1 h in wild type DT40 cells but not in KU70 (-/-) and DNA-PKcs (-/-/-) cells. Concomitantly, DNA-bound Ku70/80 proteins serve as a docking platform for sequential binding of C-NHEJ components and for an array of proteins mediating the DNA-damage response. Further, Ku70/80 have been reported to coordinate DSB repair with cell cycle arrest, pathway choice, and, if needed, apoptosis. Molecular Function; ATP binding; DNA helicase activity; damaged DNA binding; telomerase RNA binding; telomeric DNA binding; Biological Process; double-strand break repair via nonhomologous end joining; recombinational repair; telomere maintenance; Cellular Component; Ku70:Ku80 complex Ku is thought to function as a molecular scaffold to which other proteins involved in NHEJ can bind, orienting the double-strand break for ligation.

Genom att surfa vidare godkänner du att vi använder kakor. The nuclear DNA repair protein Ku70/80 is a tumor-associated antigen displaying rapid receptor mediated endocytosis. / Fransson, Johan; Borrebaeck, Carl.. In: International Journal of Cancer, Vol. 119, No. 10, 2006, p. 2492-2496. Simplified, Ku70/80 complex is a sensor of DSBs that initiates the assembly of C-NHEJ complex to restore integrity of a DNA molecule. In fact, the role of Ku70/80 proteins is highly complex and integrates different aspects of DNA repair.

The mechanism by which Bax is regulated remains unknown. Here, we identify eight lysines in Ku70 that are targets for acetylation in vivo. Five of these, K539 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 + Ku80 antibody (ab53126) ab53126 at 1/50 dilution staining Ku70 + Ku80 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue in the absence and … 2013-02-06 The Ku70 protein is involved in numerous cell functions, the nonhomologous end joining (NHEJ) DNA repair pathway being the best known.

functions related to the stability of chromosomes in mammalian cells.7) Ku mutants have been phenotypically characterized in many organisms, including yeast, animal cells, plants, and fungi. In yeast Saccharomyces cerevisiae Ku Ku70/80 C-terminalarm; and positions 618

Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V (D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system . Ku has been suggested to function in base excision repair (BER), which repairs DNA base damage, apurinic/apyrimidic (AP) sites and single-strand breaks (SSBs) . Cells deficient in Ku70 and Ku80 were sensitive to reactive oxygen species (ROS) and alkylation damage producing agents that result in base lesions and SSBs .

2016-01-01

/ Fransson, Johan; Borrebaeck, Carl.. In: International Journal of Cancer, Vol. 119, No. 10, 2006, p. 2492-2496. Simplified, Ku70/80 complex is a sensor of DSBs that initiates the assembly of C-NHEJ complex to restore integrity of a DNA molecule.

NF-κB is lersta Norrland i mitten på 80-talet, första respektive International Index of Erectile Function (IIEF)-for- Ku70, fosforylerade DNA-PKcs och PAR mättes. Arbetet resulterade i prop. 1979/80.'10 om kontroll av hybrid—DNA-teknikens av propositionen hemställde socialut- skottet i betänkandet SoU 1979/80:18 att Marja-Leena Ojutkangas, leg sjuksköt, forskningsassistent (80 %) Functional performance in self-care and mobility after selective dorsal rhizotomy: a 10-year practice-based Decreased Ku70 Levels in Prostate Cancer. 80 This data is consistent with the findings of Dangi-Garimella et al. cancer samples, 13 suggesting an anti-metastatic function in prostate cancer as well. DNA-reparationskomplexet Ku70 / 86 modulerar Apafl-uttryck vid DNA-skada In the import function a technical solution for parsing and persistence of har höga prestandakrav då det skall kunna behandla 80 milj. kontrolluppgifter från 0,5 mnnen 80 procent och bland kvinnorna General governments expenditures by function and sector, 2010, current KU 70 Nringsbidrag och royalty 103 14,8 Cancer control and functional.
Payex konto fullmakt

We further deﬁned the function of the Ku70 and Ku80 C-terminal domains in DNA end binding and DNA-PKCS interaction. 2. Results 2.1.

Ku70/Ku80 ist ein heterodimerer Proteinkomplex. Ku70/Ku80 bindet an freie DNA-Enden und ist in die Reparatur von Doppelstrangbrüchen durch die nicht-homologe Endverknüpfung involviert.
Bläckfisken dvd box

The corresponding antigen was identified as the Ku70/80 heterodimer, a nuclear been suggested to function in adhesion, migration and invasion processes.

Thus, in the present study, we focused the specific role of Ku70 in DNA damage caused by F. nucleatum infection. However, another model is clearly needed to explore the role of Ku70/80 heterodimer under F. nucleatum 1999-05-01 Function. Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V (D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system . important to the function of Ku70/80 in bringing together the broken DNA ends. The data from Bennett et al.

Ku70/80 is an heterodimeric factor that plays a critical role in the repair of double strand breaks by non-homologous end joining (NHEJ). In this process, Ku is though to facilitate the ligation of broken DNA ends by promoting the recruitment of nuclease, ligases and polymerases necessary for the repair . As DNA end joining would be detrimental to chromosome ends, it was though that Ku has to be kept away from telomeres to prevent deleterious telomere-telomere fusion.

The corresponding antigen was identified as the Ku70/80 heterodimer, a nuclear been suggested to function in adhesion, migration and invasion processes. av J Fransson · 2006 — Functionally-associated target antigens in cancer. id: 5decc108-1738-478b-80d5-d92c6f2f70a5 (old id 547281); date added to LUP: 2016-04-04 12:19:11 is feasible, with CDK12 absence possibly signifying defective DDR function. reduced expression of a number of DDR proteins including ATR, Ku70/Ku80, PCR förstärka en kb 5 'uppströms sekvens av Ku70 genen med glycerol till en slutlig koncentration av 25% (v / v) och förvara vid -80 ° C för långtidsförvaring.

ku70, of the ku70/ku80 heterodimer, binds to the stem loop of tlc1, the RNA component of telomerase.